The Influence of Ionic Environment and Histone Tails on Columnar Order of Nucleosome Core Particles.

ABSTRACT: The nucleosome core particle (NCP) is the basic building block of chromatin. Nucleosome-nucleosome interactions are instrumental in chromatin compaction, and understanding NCP self-assembly is important for understanding chromatin structure and dynamics. Recombinant NCPs aggregated by multivalent cations form various ordered phases that can be studied by x-ray diffraction (small-angle x-ray scattering). In this work, the effects on the supramolecular structure of aggregated NCPs due to lysine histone H4 tail acetylations, histone H2A mutations (neutralizing the acidic patch of the histone octamer), and the removal of histone tails were investigated. The formation of ordered mainly hexagonal columnar NCP phases is in agreement with earlier studies; however, the highly homogeneous recombinan

SUBMITTER: Berezhnoy NV 

PROVIDER: S-EPMC4850351 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature