Knockdown of ribosomal protein S15A induces human glioblastoma cell apoptosis.

ABSTRACT:

Background

RPS15A is a ribosome protein that is highly conserved in many organisms from yeast to human. A number of studies implied its role in promoting cancer cell growth.

Methods

Here, we firstly conducted RPS15A gene expression analysis in brain cancer using Oncomine database and found RPS15A was remarkably overexpressed in glioblastoma (GBM) compared with that in normal tissues. Then, the expression of RPS15A was specifically silenced in GBM cell line U251 using lentiviral-mediated RNA interference technique. We further investigated the effect of RPS15A knockdown in U251 cells using MTT assay, colony formation test, and flow cytometry analysis. We detected the protein level of Bcl-2 and poly (ADP-ribose) polymerase (PARP) as well as activation of caspase-3.

Results</

SUBMITTER: Zhang C 

PROVIDER: S-EPMC4850639 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature