Activation of a T-box-Otx2-Gsc gene network independent of TBP and TBP-related factors.

ABSTRACT: Embryonic development relies on activating and repressing regulatory influences that are faithfully integrated at the core promoter of individual genes. In vertebrates, the basal machinery recognizing the core promoter includes TATA-binding protein (TBP) and two TBP-related factors. In Xenopus embryos, the three TBP family factors are all essential for development and are required for expression of distinct subsets of genes. Here, we report on a non-canonical TBP family-insensitive (TFI) mechanism of transcription initiation that involves mesoderm and organizer gene expression. Using TBP family single- and triple-knockdown experiments, α-amanitin treatment, transcriptome profiling and chromatin immunoprecipitation, we found that TFI gene expression cannot be explained by functional redunda

SUBMITTER: Gazdag E 

PROVIDER: S-EPMC4852510 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature