Electrochemical detection of Pseudomonas in wound exudate samples from patients with chronic wounds.

ABSTRACT: In clinical practice, point-of-care diagnostic testing has progressed rapidly in the last decade. For the field of wound care, there is a compelling need to develop rapid alternatives for bacterial identification in the clinical setting, where it generally takes over 24 hours to receive a positive identification. Even new molecular and biochemical identification methods require an initial incubation period of several hours to obtain a sufficient number of cells prior to performing the analysis. Here we report the use of an inexpensive, disposable electrochemical sensor to detect pyocyanin, a unique, redox-active quorum sensing molecule released by Pseudomonas aeruginosa, in wound fluid from patients with chronic wounds enrolled in the WE-HEAL Study. By measuring the metabolite excreted by

SUBMITTER: Sismaet HJ 

PROVIDER: S-EPMC4853203 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature