Retting and degumming of natural fibers by pectinolytic enzymes produced from Bacillus tequilensis SV11-UV37 using solid state fermentation.

ABSTRACT: The present study demonstrated the simultaneous production and optimization of pectinolytic enzymes (pectate lyase and polygalacturonase) under SSF from Bacillus tequilensis SV11-UV37 using wheat bran as a substrate, which is commercially viable and cost-effective. Optimization by one variable-at-a-time-approach showed a maximum yield of pectate lyase (1371.25 U/gds) and polygalacturonase (85.45 U/gds) with wheat bran using 80 % (v/w) moisture, 0.7 mm particle size, 20 % (v/w) inoculum, 1 % (w/w) pectin at 37 °C, pH 6 and 72 h of incubation. In addition, optimization using central composite design achieved 1.6-fold improvement in both pectate lyase (1828.13 U/gds) and polygalacturonase (105.55 U/gds) yield at optimum levels of pectin (3 %, w/w), inoculum size (20 %, v/w) and moisture level

SUBMITTER: Chiliveri SR 

PROVIDER: S-EPMC4854858 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature