A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity.

ABSTRACT:

Background

Bacterial surface display is an attractive technique for the production of cell-anchored, functional proteins and engineering of whole-cell catalysts. Although various outer membrane proteins have been used for surface display, an easy and versatile high-throughput-compatible assay for evaluating and developing surface display systems is missing.

Results

Using a single domain antibody (also called nanobody) with high affinity for green fluorescent protein (GFP), we constructed a system that allows for fast, fluorescence-based detection of displayed proteins. The outer membrane hybrid protein LppOmpA and the autotransporter C-IgAP exposed the nanobody on the surface of Escherichia coli with very different efficiency. Both anchors were capable of functionally displa

SUBMITTER: Wendel S 

PROVIDER: S-EPMC4855350 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature