Live imaging of muscle histolysis in Drosophila metamorphosis.

ABSTRACT:

Background

The contribution of programmed cell death (PCD) to muscle wasting disorders remains a matter of debate. Drosophila melanogaster metamorphosis offers the opportunity to study muscle cell death in the context of development. Using live cell imaging of the abdomen, two groups of larval muscles can be observed, doomed muscles that undergo histolysis and persistent muscles that are remodelled and survive into adulthood.

Method

To identify and characterize genes that control the decision between survival and cell death of muscles, we developed a method comprising in vivo imaging, targeted gene perturbation and time-lapse image analysis. Our approach enabled us to study the cytological and temporal aspects of abnormal cell death phenotypes.

Results

In a previous g

SUBMITTER: Kuleesha Y 

PROVIDER: S-EPMC4855724 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature