Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation.

ABSTRACT:

Background

Recently, there has been a re-emergence of cutaneous leishmaniasis in endemic countries and an increase in imported cases in non-endemic countries by travelers, workers, expatriates, immigrants, and military force personnel. Old World cutaneous leishmaniasis is caused primarily by Leishmania major, L. tropica and L. aethiopica. Despite their low sensitivity, diagnosis traditionally includes microscopic and histopathological examinations, and in vitro cultivation. Several conventional PCR techniques have been developed for species identification, which are time-consuming and labour-intensive. Real-time PCR using SYBR green dye, although provides rapid detection, may generate false positive signals. Therefore, a rapid and easy method such as a FRET-based real-time PCR woul

SUBMITTER: Nath-Chowdhury M 

PROVIDER: S-EPMC4855858 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature