Gold nanorod biochip functionalization by antibody thiolation.

ABSTRACT: Conjugation of biomolecules on gold nanorod (GNR) surfaces is the basis for successful applications in biosensing, imaging, and drug delivery. Current functionalization methods are often problematic, involving multi-step nanoparticle modification to replace surfactant bilayer, delicate nanoparticle protection during surfactant exchange, and material loss due to inevitable aggregation. Instead of intensive surface modification of GNRs, we describe herein a facile method to functionalize gold nanorod surfaces via covalent Au-S bonds by thiolating receptors. The resulting GNR-bioconjugates showed superior dispersion and stability in buffer for months without morphology change and aggregation. ELISA tests confirmed the high biofunctionality of the thiolated anti-IgG moieties immobilized on the

SUBMITTER: Wang X 

PROVIDER: S-EPMC4856215 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature