Urine Exosomes for Non-Invasive Assessment of Gene Expression and Mutations of Prostate Cancer.

ABSTRACT:

Purpose

The analysis of exosome/microvesicle (extracellular vesicles (EVs)) and the RNA packaged within them (exoRNA) has the potential to provide a non-invasive platform to detect and monitor disease related gene expression potentially in lieu of more invasive procedures such as biopsy. However, few studies have tested the diagnostic potential of EV analysis in humans.

Experimental design

The ability of EV analysis to accurately reflect prostate tissue mRNA expression was examined by comparing urinary EV TMPRSS2:ERG exoRNA from pre-radical prostatectomy (RP) patients versus corresponding RP tissue in 21 patients. To examine the differential expression of TMPRSS2:ERG across patient groups a random urine sample was taken without prostate massage from a cohort of 207 men inclu

SUBMITTER: Motamedinia P 

PROVIDER: S-EPMC4856378 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature