HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behcet's Disease.

ABSTRACT:

Objective

The aim of this study was to verify the hypothesis originated from bioinformatics and literature reviews that hnNRP A1 may be a new immune target of Behçet's disease (BD).

Methods

First, bioinformatics was used to show the correlation between hnRNP A1 and A2/B1 in amino acid sequences and three dimensional structures. Second, hnRNP A1 was expressed, purified, and immunologically confirmed by systematic immunology methods including: Western blotting, immunoprecipitation and Dot-ELISA. Then, ELISA was used to screen the anti-hnRNP A1 autoantibodies in newly confirmed clinical samples and the clinical significance was compared between anti-hnRNP A1 antibody positive and negative groups. Finally, the endothelial cells antigen profile of one anti-hnRNP A1 antibody posit

SUBMITTER: Chen P 

PROVIDER: S-EPMC4856785 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature