Nucleotide polymorphisms and protein structure changes in the Fg16 gene of Fusarium graminearum sensu stricto.

ABSTRACT: Fusarium graminearum is one of the most important causes of wheat scab in different parts of the world. This fungus is able to produce widespread trichothecene mycotoxins such as nivalenol (NIV) and deoxynivalenol (DON) which are harmful for both human and animals. The Fg16 target is located in chromosome 1 of the F. graminearum genome coding for a hypothetical protein whose function is not yet known. The Fg16 gene is involved in lipid biosynthesis and leads to sexual development during colonization in wheat stalks. This gene is used to detect F. graminearum and determine the lineage of F. graminearum complex species. In the present study, polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variati

SUBMITTER: Abedi-Tizaki M 

PROVIDER: S-EPMC4856863 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature