Concordance of c-kit mutational status in matched primary and metastatic cutaneous canine mast cell tumors at baseline.

ABSTRACT:

Background

Mutation analysis of proto-oncogene c-kit (c-kit) is advisable before starting treatment with tyrosine kinase inhibitors in dogs with mast cell tumor (MCT), including those with metastatic disease. Testing is usually performed on primary tumors, assuming that c-kit mutation status does not change in metastasis.

Hypothesis/objectives

To give an insight into the mutational processes and to make a recommendation on the use of c-kit mutational analysis in the clinical setting.

Animals

Twenty-one client-owned dogs with metastatic MCT.

Methods

Dogs undergoing resection or biopsy for both primary and matched metastatic MCT were prospectively enrolled. Total RNA or DNA was extracted from primary MCT and corresponding metastases. Exons 8, 9, and 11 were ampli

SUBMITTER: Marconato L 

PROVIDER: S-EPMC4858004 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature