A Proteomics Approach to Identify New Putative Cardiac Intercalated Disk Proteins.

ABSTRACT:

Aims

Synchronous beating of the heart is dependent on the efficient functioning of the cardiac intercalated disk (ID). The ID is composed of a complex protein network enabling electrical continuity and chemical communication between individual cardiomyocytes. Recently, several different studies have shed light on increasingly prevalent cardiac diseases involving the ID. Insufficient knowledge of its composition makes it difficult to study these disease mechanisms in more detail and therefore here we aim expand the ID proteome. Here, using a combination of general membrane enrichment, in-depth quantitative proteomics and an intracellular location driven bioinformatics approach, we aim to discover new putative ID proteins in rat ventricular tissue.

Methods and results

General

SUBMITTER: Soni S 

PROVIDER: S-EPMC4858182 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature