The impact of different methods of DNA extraction on microbial community measures of BALF samples based on metagenomic data.

ABSTRACT:

Purpose

It is a challenge to find a better microorganisms DNA extraction method for samples taken from the lower airways for metagenomic sequencing, as the concentrations of bacteria in the alveoli and small airways are likely considerably less than that of the mouth or lower digestive tract. Background DNA from the host, and extraction biases can significantly interfere with microbiota assessment and increase the cost of sequencing. This study aimed to develop an optimized DNA extraction method, which would enable a higher concentration of microbial DNA to be extracted from the samples.

Methods

We compared the microbiota profiles of the lower airway communities in twelve individuals with IIP. DNA was extracted using three different extraction methods: QIAamp UCP PurePathoge

SUBMITTER: Wen Y 

PROVIDER: S-EPMC4858570 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature