Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature.

ABSTRACT: Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the struc

SUBMITTER: Tsujino S 

PROVIDER: S-EPMC4858681 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature