Simultaneous profiling of transcriptome and DNA methylome from a single cell.

ABSTRACT:

Background

Single-cell transcriptome and single-cell methylome technologies have become powerful tools to study RNA and DNA methylation profiles of single cells at a genome-wide scale. A major challenge has been to understand the direct correlation of DNA methylation and gene expression within single-cells. Due to large cell-to-cell variability and the lack of direct measurements of transcriptome and methylome of the same cell, the association is still unclear.

Results

Here, we describe a novel method (scMT-seq) that simultaneously profiles both DNA methylome and transcriptome from the same cell. In sensory neurons, we consistently identify transcriptome and methylome heterogeneity among single cells but the majority of the expression variance is not explained by proximal pr

SUBMITTER: Hu Y 

PROVIDER: S-EPMC4858893 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature