Isolation and identification of group A rotaviruses among neonatal diarrheic calves, Morocco.

ABSTRACT:

Background

Group A rotaviruses (RVA) are the main cause of neonatal calve diarrhea (NCD) in Morocco. In this study, we isolated RVA strains from NCD clinical samples in order to support RVA disease control in Morocco. This isolation process constitutes a first step toward vaccine development.

Methods

Thirteen fecal samples were obtained from calves with a single episode of neonate calf diarrhea at three different dairies and two samples were collected from field during a severe NCD outbreak. Diagnosis of RVA infection was based on fecal immune-chromatographic rapid test and further evaluated for their hemagglutination (HA) activity. RVA isolation was carried out on MA104 cells after inoculates were treated with different concentrations of trypsin TPCK. All RVA isolates were

SUBMITTER: Ennima I 

PROVIDER: S-EPMC4858901 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature