Differential expression of conserved and novel microRNAs during tail regeneration in the lizard Anolis carolinensis.

ABSTRACT:

Background

Lizards are evolutionarily the most closely related vertebrates to humans that can lose and regrow an entire appendage. Regeneration in lizards involves differential expression of hundreds of genes that regulate wound healing, musculoskeletal development, hormonal response, and embryonic morphogenesis. While microRNAs are able to regulate large groups of genes, their role in lizard regeneration has not been investigated.

Results

MicroRNA sequencing of green anole lizard (Anolis carolinensis) regenerating tail and associated tissues revealed 350 putative novel and 196 known microRNA precursors. Eleven microRNAs were differentially expressed between the regenerating tail tip and base during maximum outgrowth (25 days post autotomy), including miR-133a, miR-133b, and

SUBMITTER: Hutchins ED 

PROVIDER: S-EPMC4858913 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature