Natural Genetic Variation Differentially Affects the Proteome and Transcriptome in Caenorhabditis elegans.

ABSTRACT: Natural genetic variation is the raw material of evolution and influences disease development and progression. An important question is how this genetic variation translates into variation in protein abundance. To analyze the effects of the genetic background on gene and protein expression in the nematode Caenorhabditis elegans, we quantitatively compared the two genetically highly divergent wild-type strains N2 and CB4856. Gene expression was analyzed by microarray assays, and proteins were quantified using stable isotope labeling by amino acids in cell culture. Among all transcribed genes, we found 1,532 genes to be differentially transcribed between the two wild types. Of the total 3,238 quantified proteins, 129 proteins were significantly differentially expressed between N2 and CB4856.

SUBMITTER: Kamkina P 

PROVIDER: S-EPMC4858947 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature