Glycosylated gold nanoparticle libraries for label-free multiplexed lectin biosensing.

ABSTRACT: Glycan/lectin interactions drive a wide range of recognition and signal transduction processes within nature. However, their measurement is complicated or limited by the analytical tools available. Most technologies require fluorescently labelled proteins (e.g. microarrays) or expensive infrastructure (such as surface plasmon resonance). This also limits their application in biosensing, especially for low-resource settings, where detection of pathogens based on glycan binding could speed up diagnosis. Here we employ a library-oriented approach to immobilise a range of monosaccharides onto polymer-stabilised gold nanoparticles to enable rapid and high-throughput evaluation of their binding specificities with a panel of lectins. The red to blue colour shift upon gold nanoparticle aggr

SUBMITTER: Richards SJ 

PROVIDER: S-EPMC4859411 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature