Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis.

ABSTRACT: The development of precision medicine, together with the multiplication of targeted therapies and associated molecular biomarkers, call for major progress in genetic analysis methods, allowing increased multiplexing and the implementation of more complex decision trees, without cost increase or loss of robustness. We present a platform combining droplet microfluidics and magnetic tweezers, performing RNA purification, reverse transcription and amplification in a fully automated and programmable way, in droplets of 250nL directly sampled from a microtiter-plate. This platform decreases sample consumption about 100 fold as compared to current robotized platforms and it reduces human manipulations and contamination risk. The platform's performance was first evaluated on cell lines, showing ro

SUBMITTER: Ferraro D 

PROVIDER: S-EPMC4860594 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature