Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.

ABSTRACT: Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a powerful genome-editing method that facilitates genetic alterations of genomes in a variety of organisms. However, a genome-editing approach has not been reported in filamentous fungi. Here, we demonstrated the establishment of a CRISPR/Cas9 system in the filamentous fungus Trichoderma reesei by specific codon optimization and in vitro RNA transcription. It was shown that the CRISPR/Cas9 system was controllable and conditional through inducible Cas9 expression. This system generated site-specific mutations in target genes through efficient homologous recombination, even using short homology arms. This system also provided an applicable and promising approach to targeting multiple genes simultaneously. Ou

SUBMITTER: Liu R 

PROVIDER: S-EPMC4860831 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature