Screening for MIR184 Mutations in Iranian Patients with Keratoconus.

ABSTRACT:

Purpose

To investigate whether microRNA (MIR)-184 mutations make a substantial contribution to keratoconus (KCN) among affected Iranian patients.

Methods

A total of 47 Iranian KCN patients, diagnosed based on family history, clinical examinations using slit lamp biomicroscopy, refraction and corneal topography were enrolled in this study. The pri-miR-184 encoding gene obtained from the DNAs of all participants was amplified using polymerase chain reaction and subsequently sequenced by the Sanger dideoxynucleotide protocol. The sequences were compared to MIR184 reference sequence in order to identify sequence variations. The potential effects of a single variation observed on RNA structure was predicted.

Results

Only one sequence variation, +39G >T, was observed within

SUBMITTER: Farzadfard A 

PROVIDER: S-EPMC4860983 | biostudies-literature | 2016 Jan-Mar

REPOSITORIES: biostudies-literature