Kinetic Study of Yellow Fever 17DD Viral Infection in Gallus gallus domesticus Embryos.

ABSTRACT: Yellow fever continues to be an important epidemiological problem in Africa and South America even though the disease can be controlled by vaccination. The vaccine has been produced since 1937 and is based on YFV 17DD chicken embryo infection. However, little is known about the histopathological background of virus infection and replication in this model. Here we show by morphological and molecular methods (brightfield and confocal microscopies, immunofluorescence, nested-PCR and sequencing) the kinetics of YFV 17DD infection in chicken embryos with 9 days of development, encompassing 24 to 96 hours post infection. Our principal findings indicate that the main cells involved in virus production are myoblasts with a mesenchymal shape, which also are the first cells to express virus proteins

SUBMITTER: Manso PP 

PROVIDER: S-EPMC4861264 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature