Age-Dependent Susceptibility to Enteropathogenic Escherichia coli (EPEC) Infection in Mice.

ABSTRACT: Enteropathogenic Escherichia coli (EPEC) represents a major causative agent of infant diarrhea associated with significant morbidity and mortality in developing countries. Although studied extensively in vitro, the investigation of the host-pathogen interaction in vivo has been hampered by the lack of a suitable small animal model. Using RT-PCR and global transcriptome analysis, high throughput 16S rDNA sequencing as well as immunofluorescence and electron microscopy, we characterize the EPEC-host interaction following oral challenge of newborn mice. Spontaneous colonization of the small intestine and colon of neonate mice that lasted until weaning was observed. Intimate attachment to the epithelial plasma membrane and microcolony formation were visualized only in the presence of a functio

SUBMITTER: Dupont A 

PROVIDER: S-EPMC4861285 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature