Action of nicotinic acid on the reversion of hypoxic-inflammatory link on 3T3-L1 adipocytes.

ABSTRACT:

Background

Hypoxia resulting from adipocyte expansion is considered the basis of the inflammatory milieu observed in Metabolic Syndrome. Nicotinic acid can act on adipocytes interfering on the inflammatory response. In this study, we investigated the role of HIF-1 α (hypoxia-inducible factor -1 alpha) in the inflammatory process induced by hypoxia. The effect of nicotinic acid on the PPARs (peroxisome proliferator-activated receptors) expression during the inflammatory response was assessed over its action under HIF-1 α in 3T3-L1 adipocytes submitted to hypoxia.

Methods

3T3-L1 adipocytes were pre-treated with nicotinic acid and incubated under hypoxic conditions. The level of adipokines and HIF-1 α were quantified using immunoassays. Adipokine expression was measured using r

SUBMITTER: Nakamichi R 

PROVIDER: S-EPMC4862071 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature