Estimation of ribosome profiling performance and reproducibility at various levels of resolution.

ABSTRACT:

Background

Ribosome profiling (or Ribo-seq) is currently the most popular methodology for studying translation; it has been employed in recent years to decipher various fundamental gene expression regulation aspects. The main promise of the approach is its ability to detect ribosome densities over an entire transcriptome in high resolution of single codons. Indeed, dozens of ribo-seq studies have included results related to local ribosome densities in different parts of the transcript; nevertheless, the performance of Ribo-seq has yet to be quantitatively evaluated and reported in a large-scale multi-organismal and multi-protocol study of currently available datasets.

Results

Here we provide the first objective evaluation of Ribo-seq at the resolution of a single nucleotide(

SUBMITTER: Diament A 

PROVIDER: S-EPMC4862193 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature