Network Analysis of Sequence-Function Relationships and Exploration of Sequence Space of TEM β-Lactamases.

ABSTRACT: The Lactamase Engineering Database (www.LacED.uni-stuttgart.de) was developed to facilitate the classification and analysis of TEM β-lactamases. The current version contains 474 TEM variants. Two hundred fifty-nine variants form a large scale-free network of highly connected point mutants. The network was divided into three subnetworks which were enriched by single phenotypes: one network with predominantly 2be and two networks with 2br phenotypes. Fifteen positions were found to be highly variable, contributing to the majority of the observed variants. Since it is expected that a considerable fraction of the theoretical sequence space is functional, the currently sequenced 474 variants represent only the tip of the iceberg of functional TEM β-lactamase variants which form a huge natural r

SUBMITTER: Zeil C 

PROVIDER: S-EPMC4862526 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature