Aberrant Calreticulin Expression in Articular Cartilage of Dio2 Deficient Mice.

ABSTRACT:

Objective

To identify intrinsic differences in cartilage gene expression profiles between wild-type- and Dio2-/--mice, as a mechanism to investigate factors that contribute to prolonged healthy tissue homeostasis.

Methods

Previously generated microarray-data (Illumina MouseWG-6 v2) of knee cartilage of wild-type and Dio2 -/- -mice were re-analyzed to identify differential expressed genes independent of mechanical loading conditions by forced treadmill-running. RT-qPCR and western blot analyses of overexpression and knockdown of Calr in mouse chondro-progenitor cells (ATDC5) were applied to assess the direct effect of differential Calr expression on cartilage deposition.

Results

Differential expression analyses of articular cartilage of Dio2-/- (N = 9) and wild-type-mi

SUBMITTER: Bomer N 

PROVIDER: S-EPMC4862667 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature