Validation of a DNA methylation microarray for 850,000 CpG sites of the human genome enriched in enhancer sequences.

ABSTRACT:

Aim

DNA methylation is the best known epigenetic mark. Cancer and other pathologies show an altered DNA methylome. However, delivering complete DNA methylation maps is compromised by the price and labor-intensive interpretation of single nucleotide methods.

Material & methods

Following the success of the HumanMethylation450 BeadChip (Infinium) methylation microarray (450K), we report the technical and biological validation of the newly developed MethylationEPIC BeadChip (Infinium) microarray that covers over 850,000 CpG methylation sites (850K). The 850K microarray contains >90% of the 450K sites, but adds 333,265 CpGs located in enhancer regions identified by the ENCODE and FANTOM5 projects.

Results & conclusion

The 850K array demonstrates high reproducibility at the

SUBMITTER: Moran S 

PROVIDER: S-EPMC4864062 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature