Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human β Cell Function.

ABSTRACT: Intensive efforts are focused on identifying regulators of human pancreatic islet cell growth and maturation to accelerate development of therapies for diabetes. After birth, islet cell growth and function are dynamically regulated; however, establishing these age-dependent changes in humans has been challenging. Here, we describe a multimodal strategy for isolating pancreatic endocrine and exocrine cells from children and adults to identify age-dependent gene expression and chromatin changes on a genomic scale. These profiles revealed distinct proliferative and functional states of islet α cells or β cells and histone modifications underlying age-dependent gene expression changes. Expression of SIX2 and SIX3, transcription factors without prior known functions in the pancreas and linked t

SUBMITTER: Arda HE 

PROVIDER: S-EPMC4864151 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature