Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.

ABSTRACT: Catenation links between sister chromatids are formed progressively during DNA replication and are involved in the establishment of sister chromatid cohesion. Topo IV is a bacterial type II topoisomerase involved in the removal of catenation links both behind replication forks and after replication during the final separation of sister chromosomes. We have investigated the global DNA-binding and catalytic activity of Topo IV in E. coli using genomic and molecular biology approaches. ChIP-seq revealed that Topo IV interaction with the E. coli chromosome is controlled by DNA replication. During replication, Topo IV has access to most of the genome but only selects a few hundred specific sites for its activity. Local chromatin and gene expression context influence site selection. Moreover str

SUBMITTER: El Sayyed H 

PROVIDER: S-EPMC4865107 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature