Overexpression of Sly41 suppresses COPII vesicle-tethering deficiencies by elevating intracellular calcium levels.

ABSTRACT: SLY41 was identified as a multicopy suppressor of loss of Ypt1, a Rab GTPase essential for COPII vesicle tethering at the Golgi complex. SLY41 encodes a polytopic membrane protein with homology to a class of solute transporter proteins, but how overexpression suppresses vesicle-tethering deficiencies is not known. Here we show that Sly41 is efficiently packaged into COPII vesicles and actively cycles between the ER and Golgi compartments. SLY41 displays synthetic negative genetic interactions with PMR1, which encodes the major Golgi-localized Ca(2+)/Mn(2+) transporter and suggests that Sly41 influences cellular Ca(2+) and Mn(2+) homeostasis. Experiments using the calcium probe aequorin to measure intracellular Ca(2+) concentrations in live cells reveal that Sly41 overexpression significant

SUBMITTER: Mukherjee I 

PROVIDER: S-EPMC4865320 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature