Regulation of the T-box transcription factor Tbx3 by the tumour suppressor microRNA-206 in breast cancer.

ABSTRACT:

Background

The Tbx3 transcription factor is over-expressed in breast cancer, where it has been implicated in proliferation, migration and regulation of the cancer stem cell population. The mechanisms that regulate Tbx3 expression in cancer have not been fully explored. In this study, we demonstrate that Tbx3 is repressed by the tumour suppressor miR-206 in breast cancer cells.

Methods

Bioinformatics prediction programmes and luciferase reporter assays were used to demonstrate that miR-206 negatively regulates Tbx3. We examined the impact of miR-206 on Tbx3 expression in breast cancer cells using miR-206 mimic and inhibitor. Gene/protein expression was examined by quantitative reverse-transcription-PCR and immunoblotting. The effects of miR-206 and Tbx3 on apoptosis, prolifer

SUBMITTER: Amir S 

PROVIDER: S-EPMC4865973 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature