Regulation of E3 ubiquitin ligase-1 (WWP1) by microRNA-452 inhibits cancer cell migration and invasion in prostate cancer.

ABSTRACT:

Background

MicroRNA-224 (miR-224) and microRNA-452 (miR-452) are closely located on the human chromosome Xq28 region. miR-224 functions as a tumour suppressor by targeting tumour protein D52 (TPD52) in prostate cancer (PCa). Here, we aimed to investigate the functional significance of miR-452 in PCa cells.

Methods

Functional studies of PCa cells were performed using transfection with mature miRNAs or siRNAs. Genome-wide gene expression analysis, in silico analysis, and dual-luciferase reporter assays were applied to identify miRNA targets. The association between miR-452 levels and overall patient survival was estimated by the Kaplan-Meier method.

Results

Expression of miR-452 was significantly downregulated in PCa tissues. Transfection with mature miR-452 inhibited t

SUBMITTER: Goto Y 

PROVIDER: S-EPMC4865980 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature