Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer.

ABSTRACT:

Background

Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer.

Methods

Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expression in human pancreatic tumors. Then short hairpin RNA (shRNA) targeting ZFR was designed and delivered into PANC-1 pancreatic cancer cells to knock down ZFR expression. Cell viability, cell proliferation and cell cycle analysis after ZFR knockdown were determined by MTT, colony forming and FACS, respectively. In addition, cell migration and invasion were assessed using the Transwell system.

Results

The expression of ZFR was significantly higher in panc

SUBMITTER: Zhao X 

PROVIDER: S-EPMC4866406 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature