Molecular identification and phylogenic analysis of Bartonella henselae isolated from Iranian cats based on gltA gene.

ABSTRACT: One of the most important species of the Bartonella genus is B. henselae that causes a zoonotic infection, cat scratch disease (CSD). The main source of the bacteria is cat and the carrier is Ctenocephalides felis flea. One hundred and forty nail and saliva samples were collected from 70 domestic cats. Positive samples for B. henselae were characterized by polymerase chain reaction (PCR) and sequencing. Sequences of gltA gene were trimmed using BioEdit software and then compared with the sequences of the same gene from B. henselae isolated from cats and humans in GenBank database. Phylogenic tree was constructed using CLC Sequence Viewer software and unweighted pair group method with arithmetic mean (UPGMA) method. Molecular assessments showed that five samples out of 70 nail samples (7.14

SUBMITTER: Mazaheri Nezhad Fard R 

PROVIDER: S-EPMC4867040 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature