Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry.

ABSTRACT:

Aim

To analyze the lipid distribution in gastric mucosae.

Methods

Imaging mass spectrometry (MS) is a useful tool to survey the distribution of biomolecules in surgical specimens. Here we used the imaging MS apparatus named iMScope to identify the dominant molecules present in the human gastric mucosa near the fundic glands. Five gastric specimens were subjected to iMScope analysis. These specimens were also analyzed by immunohistochemistry using MUC5AC, H(+)-K(+)-ATPaseβ Claudin18 antibodies.

Results

Three major molecules with m/z 725.5, 780.5, and 782.5 detected in the gastric mucosa were identified as sphingomyelin (SM) (d18:1/16:0), phosphatidylcholine (PC) (16:0/18:2), and PC (16:0/18:1), respectively, through MS/MS analyses. Using immunohistological staining, SM

SUBMITTER: Kurabe N 

PROVIDER: S-EPMC4867403 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature