Rapid, scalable, and low-cost purification of recombinant adeno-associated virus produced by baculovirus expression vector system.

ABSTRACT: Recombinant adeno-associated viruses (rAAV) are largely used for gene transfer in research, preclinical developments, and clinical trials. Their broad in vivo biodistribution and long-term efficacy in postmitotic tissues make them good candidates for numerous gene transfer applications. Upstream processes able to produce large amounts of rAAV were developed, particularly those using baculovirus expression vector system. In parallel, downstream processes present a large panel of purification methods, often including multiple and time consuming steps. Here, we show that simple tangential flow filtration, coupled with an optimized iodixanol-based isopycnic density gradient, is sufficient to purify several liters of crude lysate produced by baculovirus expression vector system in only one work

SUBMITTER: Buclez PO 

PROVIDER: S-EPMC4867670 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature