Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay.

ABSTRACT:

Background

Leishmania donovani (LD) is a protozoan parasite transmitted to humans from sand flies, which causes Visceral Leishmaniasis (VL). Currently, the diagnosis is based on presence of the anti-LD antibodies and clinical symptoms. Molecular diagnosis would require real-time PCR, which is not easy to implement at field settings. In this study, we report on the development and testing of a recombinase polymerase amplification (RPA) assay for the detection of LD.

Methods

A genomic DNA sample was applied to determine the assay analytical sensitivity. The cross-reactivity of the assay was tested by DNA of Leishmania spp. and of pathogens considered for differential diagnosis. The clinical performance of the assay was evaluated on LD positive and negative samples. All results

SUBMITTER: Mondal D 

PROVIDER: S-EPMC4868004 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature