Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses.

ABSTRACT:

Purpose

We characterize calpain-5 (CAPN5) expression in retinal and neuronal subcellular compartments.

Methods

CAPN5 gene variants were classified using the exome variant server, and RNA-sequencing was used to compare expression of CAPN5 mRNA in the mouse and human retina and in retinoblastoma cells. Expression of CAPN5 protein was ascertained in humans and mice in silico, in mouse retina by immunohistochemistry, and in neuronal cancer cell lines and fractionated central nervous system tissue extracts by Western analysis with eight antibodies targeting different CAPN5 regions.

Results

Most CAPN5 genetic variation occurs outside its protease core; and searches of cancer and epilepsy/autism genetic databases found no variants similar to hyperactivating retinal disease a

SUBMITTER: Schaefer KA 

PROVIDER: S-EPMC4868102 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature