A Simple Method for Optimization of Reference Gene Identification and Normalization in DNA Microarray Analysis.

ABSTRACT:

Background

Comparative DNA microarray analyses typically yield very large gene expression data sets that reflect complex patterns of change. Despite the wealth of information that is obtained, the identification of stable reference genes is required for normalization of disease- or drug-induced changes across tested groups. This is a prerequisite in quantitative real-time reverse transcription-PCR (qRT-PCR) and relative RT-PCR but rare in gene microarray analysis. The goal of the present study was to outline a simple method for identification of reliable reference genes derived from DNA microarray data sets by comparative statistical analysis of software-generated and manually calculated candidate genes.

Material and methods

DNA microarray data sets derived from whole-blood

SUBMITTER: Casares FM 

PROVIDER: S-EPMC4868104 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature