Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue.

ABSTRACT: Calcium-imaging is a sensitive method for monitoring calcium dynamics during neuronal activity. As intracellular calcium concentration is correlated to physiological and pathophysiological activity of neurons, calcium imaging with fluorescent indicators is one of the most commonly used techniques in neuroscience today. Current methodologies for loading calcium dyes into the tissue require prolonged incubation time (45-150 min), in addition to dissection and recovery time after the slicing procedure. This prolonged incubation curtails experimental time, as tissue is typically maintained for 6-8 hours after slicing. Using a recently introduced recovery chamber that extends the viability of acute brain slices to more than 24 hours, we tested the effectiveness of calcium AM staining following

SUBMITTER: Cameron M 

PROVIDER: S-EPMC4868260 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature