A tyrosine phosphatase SHP2 gain-of-function mutation enhances malignancy of breast carcinoma.

ABSTRACT:

Background

Evidence suggests that Src homologous protein phosphotyrosyl phosphatase 2 (SHP2) mutations promote cancer development in several solid tumours. In this study, we focused on the in vivo and in vitro effects of an SHP2 mutation on the breast cancer phenotype to determine whether this mutation is correlated with a malignant phenotype.

Methods

Mutant PTPN11 cDNA (D61G) was transduced into MDA-MB231 and MCF-7 cells. The effects of the D61G mutation on tumourigenesis and malignant behaviours, such as cell adhesion, proliferation, migration and invasion, were examined. Potential underlying molecular mechanisms, i.e., activation of the Gab1-Ras-Erk axis, were also examined.

Results

In vitro experiments revealed that tumour adhesion, proliferation, migration and in

SUBMITTER: Hu Z 

PROVIDER: S-EPMC4868712 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature