Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.

ABSTRACT: Protein array technology is a powerful platform for the simultaneous determination of the expression levels of a number of proteins as well as post-translational modifications such as phosphorylation. Here, we screen and report for the first time, the dominant signaling cascades and apoptotic mediators during the course of cadmium (Cd)-induced cytotoxicity in human bronchial epithelial cells (BEAS-2B) by antibody array analyses. Proteins from control and Cd-treated cells were captured on Proteome Profiler™ Arrays for the parallel determination of the relative levels of protein phosphorylation and proteins associated with apoptosis. Our results indicated that the p38 MAPK- and JNK-related signal transduction pathways were dramatically activated by Cd treatment. Cd potently stimulates the ph

SUBMITTER: Xu YM 

PROVIDER: S-EPMC4868746 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature