BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia.

ABSTRACT:

Background

Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem.

Results

We proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous form

SUBMITTER: Cordeiro M 

PROVIDER: S-EPMC4869199 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature