RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.

ABSTRACT:

Background

Rare cell subtypes can profoundly impact the course of human health and disease, yet their presence within a sample is often missed with bulk molecular analysis. Single-cell analysis tools such as FACS, FISH-FC and single-cell barcode-based sequencing can investigate cellular heterogeneity; however, they have significant limitations that impede their ability to identify and transcriptionally characterize many rare cell subpopulations.

Results

PCR-activated cell sorting (PACS) is a novel cytometry method that uses single-cell TaqMan PCR reactions performed in microfluidic droplets to identify and isolate cell subtypes with high-throughput. Here, we extend this method and demonstrate that PACS enables high-dimensional molecular profiling on TaqMan-targeted cells. Us

SUBMITTER: Pellegrino M 

PROVIDER: S-EPMC4869385 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature