Extreme obesity induces massive beta cell expansion in mice through self-renewal and does not alter the beta cell lineage.

ABSTRACT:

Aims/hypothesis

Understanding the developmental biology of beta cell regeneration is critical for developing new diabetes therapies. Obesity is a potent but poorly understood stimulus for beta cell expansion. Current models of obesity are complicated by developmental compensation or concurrent diabetes, limiting their usefulness for identifying the lineage mechanism(s) of beta cell expansion. We aimed to determine whether acute inducible obesity stimulates beta cell expansion and to determine the lineage mechanism of beta cell growth in obesity.

Methods

We created whole-body tamoxifen-inducible leptin receptor (LepR)-deficient mice (Ubc-Cre (ERT2) LepR (loxP/loxP) ) as a novel model of acute obesity. Beta cell mass and proliferation were quantified after short-term LepR dele

SUBMITTER: Cox AR 

PROVIDER: S-EPMC4869735 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature