Persistent Gliosis Interferes with Neurogenesis in Organotypic Hippocampal Slice Cultures.

ABSTRACT: Neurogenesis in the adult hippocampus has become an intensively investigated research topic, as it is essential for proper hippocampal function and considered to bear therapeutic potential for the replacement of pathologically lost neurons. On the other hand, neurogenesis itself is frequently affected by CNS insults. To identify processes leading to the disturbance of neurogenesis, we made use of organotypic hippocampal slice cultures (OHSC), which, for unknown reasons, lose their neurogenic potential during cultivation. In the present study, we show by BrdU/Prox1 double-immunostaining that the generation of new granule cells drops by 90% during the first week of cultivation. Monitoring neurogenesis dynamically in OHSC from POMC-eGFP mice, in which immature granule cells are endogenously l

SUBMITTER: Gerlach J 

PROVIDER: S-EPMC4870256 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature